CHROMABOND® SiOH-H2SO4/SA | Volume ml: 3

* one filter element is already inserted in the column.

Extraction and clean-up mixtures for sample preparation in the pesticide analysis of vegetable foodstuffs according to EN 15662 using GC/MS or LC/MS after acetonitrile extraction and purification using the dispersive SPE-QuEChERS method.The samples are first extracted with Mix A. Purification is done with one of the following mixtures:Mix B (Clean-up mixture for fruits and vegetables with low fat content, e.g. apples, strawberries)Mix C (Clean-up mixture for fruits and vegetables with high fat content, containing oil/wax, e.g. avocados)Mix D (Clean-up mixture for moderately chlorophyll/carotenoid-containing fruits and vegetables, e.g. carrots, salad)Mix E (Clean-up mixture for fruits and vegetables with high chlorophyll/carotenoid content, e.g. sweet peppers, spinach)If required, add ceramic homogenising beads for better mixing.Mix A contains a bag with the required amount of mixture and a 50 ml empty tubeMix B, C, D and E are pre-weighed into a 15 ml tube

The innovative cation exchanger offers:mixed-mode SPE phase with distinct cation exchange and RP properties excellent enrichment of basic analytesmodern support polymer with optimised pore structure and large specific surfacegood reproducibility, reliable and cost-efficient analysesmore aggressive washing procedures for matrix removal possiblecleaner samples and protection of analytical HPLC and GC instrumentationquantificationof analytes from heavily contaminated sampleslower limits of detection also for critical matrices... the perfect fit for basic analytes in comination with CHROMABOND® HR-X

Polar modified polystyrene-divinylbenzene copolymer with a weak anion exchangerSpecific surface 650 to 700 m²/g, particle size 80µm, pore size 50 Å, pH stability 1 to 14. Due to bifunctional modification much more hydrophilic than conventional polystyrene-divinylbenzene polymers and thus easily wettable with water.Recommended applications: polar herbicides / pesticides from water (acidic, neutral, basic), polar phenols from water, polyaromatic compounds, polychlorinated biphenyls, drug analysis from urine, blood, serum, plasma pharmaceuticals / active ingredients from tablets, creams.Due to the bifunctional modification CHROMABOND® Easy is considerably more hydrophilic than conventional polystyrene-divinylbenzene polymers and thus easily wettable with water. The Easy effect: Aqueous samples can be loaded directly without preconditioning! This means that little or even no conditioning is needed, in contrast to standard SPE materials, where recovery rates normally decrease, in the worst case down to zero! Depending on the separation task conditioning may be required and is recommended for method development. A positive side effect of the excellent wettability: there is no decrease of recovery rates, if the cartridge runs dry, therefore automation is easier or, in some cases compared to silica materials, only feasible with CHROMABOND® Easy, because a permanent vacuum can be used without supervision.Further advantages of using a polymeric material:high surface, this means very high binding capacity (2 - 5 times higher than silica-based adsorbents)less adsorbent is needed in the cartridge (without losing sensitivity or recovery)faster analysis, because the height of the adsorbent bed can be reducedacidic or basic solvents (e. g. TFA) do not destroy the phase, or lead to unintended side productsBecause of the polar modification the material is suitable for a broad range of compounds (acidic, neutral, basic, polar and nonpolar substances). Highly reproducible recovery rates can be obtained, even if the cartridge runs dry (especially advantageous when using 96-well plates, where stopcocks are not available!)BIGpacks: 4.003 723 / 4.003 724

Spherical, hydrophobic polystyrene-divinylbenzene resin for SPEHydrophobic polystyrene-divinylbenzene copolymer pH stability 1 to14. High-purity material with highest reproducibility and lowest blank values due to a novel manufacturing process, spherical particles 85mm; pore size 55 to 60Å. Very high surface 1000m²/g; capacity 390mg/g (caffeine in water). Excellent recovery rates especially for the enrichment of pharmaceuticals/active ingredients due to the spherical structure of the particles, very homogeneous surface, and optimised pore structure.Recommended applications: pharmaceuticals/active ingredients from tablets, creams and water/waste water, drugs and pharmaceuticals from urine, blood, serum and plasma trace analysis of pesticides.Cartridges in further sizes and phases of the HR-X poduct range available on request.

The innovative cation exchanger offers:mixed-mode SPE phase with distinct cation exchange and RP properties excellent enrichment of basic analytesmodern support polymer with optimised pore structure and large specific surfacegood reproducibility, reliable and cost-efficient analysesmore aggressive washing procedures for matrix removal possiblecleaner samples and protection of analytical HPLC and GC instrumentationquantificationof analytes from heavily contaminated sampleslower limits of detection also for critical matrices... the perfect fit for basic analytes in comination with CHROMABOND® HR-XBenzenesulfonic acid modified polymeric cation exchanger for SPE Base material spherical PS/DVB copolymer, pore size 65-75Å, particle sizes 45µm or 85µm (standard), very large specific surface 800m²/g, pore volume 1.4cm³/g, pH stability 1-14. Benzenesulfonic acid modification, exchange capacity 1.0meq/g, pKa < 1 RP capacity 300mg/g (caffeine in water). High-purity material, highest reproducibility and lowest blank values due to an optimized production process, outstanding recovery rates especially for the enrichment of basic analytes for standard protocol.Recommended application: basic active ingredients from strongly matrix-contaminated samples like, e.g., urine, plasma, serum, fungicides from food, basic analytes like, e.g., amines, bases with pKa 2-10.

QuEChERS method and pre-mixesWithin a few years after its devleopment by Anastassiades et al. the QuEChERS method has gained a leading position for determination of pesticide residues in food by GC-MS or LC-MS allowing rapid and cheap clean-up of strong matrix-contaminated samples.Standard clean-up of food samples10 g sample are homogenised with 10 ml acetonitrile. After adding the internal standard the sample is shaken with 4 g MgSO4 and 1 g NaCI and afterwards centrifuged. 1 ml of the supernantant is spiked with 25 mg CHROMABOND® Diamino and 150 mg MgSO4 and shaken again. After centrifugation the supernatant is injected into the GC/MS.Extraction mixtures for sample preparation for determination of pesticides in food samples.MACHEREY-NAGEL offers a number of individually weighed and pre-mixed buffers and extraction mixtures, specially composed for different sample matrices.Mix I and Mix II are extraction mixtures, Mix III to Mix VI are clean-up mixtures.The food samples will be extracted with either Mix I or Mix II.Afterwards they will be purified with one of the following mixtures:Mix III (samples with low fat content; e.g. apples, strawberries),Mix IV (moderate content of chlorophyll and carotinoids; e. g. carrots, lettuce),Mix V (high content of chlorophyll and carotinoids; e. g. bell peppers, spinach),Mix VI (high fat content; e. g. avocados).Every mix is prepacked in a 15 ml centrifuge tube. Pack of 50 pieces.

Octadecyl modified silica phase for SPE, not endcappedBase material silica, pore size 60 Å, particle size 45 µm for C18, specific surface 500 m²/g, pH stability 2 to 8 octadecyl phases, not endcapped, carbon content 14 % possesses more free silanols (SiOH), which allow secondary interactions with polar groups of the analytes.Recommended applications: non-polar compounds, pesticides.

Octadecyl modified silica phase for SPE, endcappedBase material silica, pore size 60 Å, particle size 45 µm for C18 ec, 100 µm for C18 ec f (for fast flow), specific surface 500 m²/g, pH stability 2 to 8. Octadecyl phases, endcapped, carbon content 14 % very non-polar, hydrophobic interactions with a wide variety of organic compounds. Advantageous for clean-up of samples with large structural variations (polarity differences).Recommended applications: non-polar compounds, aflatoxins, amphetamines, antibiotics, antiepileptics, barbiturates, caffeine, drugs, preservatives, fatty acids, nicotine, PAHs, pesticides, PCBs, heavy metals, vitamins. Very well suited for desalting of samples.BIGpacks: 4.003 446 / 4.003 450 / 4.003 452

Octyl modified silica phase for SPE, not endcappedBase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8. Octyl phase, not endcapped, carbon content 8%, similar to C18, however slightly more polar. Secondary interactions with polar compounds are more pronounced due to shorter alkyl chains.Recommended applications: pesticides, PCBs.