Electroporation system ECM® 830 | Type: ECM® 830

BTX Cuvettes Plus are designed for use in electroporation and electrofusion of bacteria, yeast, insect, plant and mammalian cells. Each sterilized Cuvettes Plus package includes a disposable cuvette and a transfer pipette, which is used for removal of the sample after electroporation. The cuvettes are molded with embedded polished aluminum electrodes, and gamma irradiated for guaranteed sterility.Three electrode gap sizes are available:1mm for bacteria and yeast2mm for all cell types4mm for mammalian cellsBTX cuvette Plus can obtain high field strengths up to 25.0 kV/cm. Round caps are leak resistant and allow for quick and easy removal. For research use only. Can be used with electroporation systems from different manufacturers (not suitable for units from Eppendorf).

The Gemini Twin Wave devices are flexible systems that can generate both square wave and exponential decay waves. Ideally suited for all electroporation requirements. The waveform combination allows eukaryotic and prokaryotic cells to be electroporated easily and efficiently in cuvettes or plates with a single device. Prokaryotic cells typically respond well to exponential decay wave pulses, while eukaryotic cells are most efficiently transfected with square wave pulses.Large, user-friendly touchscreen interfaceUniversal electroporation for transfection of cells in vitro, in vivo, in ovo, and adherent formsPreset protocols include the most common eukaryotic and prokaryotic cell typesUser-defined protocols: protocols can be added and modified without limitsSafety functions: Pre-pulse resistance check, arc protection, and over-current pulse abortData protocols store parameters of all delivered pulses for quality control and troubleshooting purposesStorage of over 1000 protocolsScope of supply: Generator Gemini X2Exponential decay pulseCapacitance LV mode:25 ... 3275 µFCapacitance HV mode:10, 25, 35, 50, 60, 75, 85 µFResistance LV mode:25 ... 1575 ΩResistance HV mode:50 ... 1575 ΩVoltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse:1 ... 2 (depending on amplitude limit of the device)Pulse interval:5 ... 30 sSquare wave pulseCapacitance LV mode:3775 µFCapacitance HV mode:85 µFVoltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse single sample:1 ... 99 pulses per sampleMultiple pulse plate:1 ... 10 pulses per samplePulse length LV mode:10 µs ... 999 msPulse length HV mode:10 ... 600 µsPulse interval:0.1 ... 10 sCharging time LV mode:7 sCharging time HV mode:4 s

The Gemini Twin Wave devices are flexible systems that can generate both square wave and exponential decay waves. Ideally suited for all electroporation requirements. The waveform combination allows eukaryotic and prokaryotic cells to be electroporated easily and efficiently in cuvettes with a single device. Prokaryotic cells typically respond well to exponential decay wave pulses, while eukaryotic cells are most efficiently transfected with square wave pulses.Large, user-friendly touchscreen interfaceUniversal electroporation for transfection of cells in vitro, in vivo, in ovo, and adherent formsPreset protocols include the most common eukaryotic and prokaryotic cell typesUser-defined protocols: protocols can be added and modified without limitsSafety functions: Pre-pulse resistance check, arc protection, and over-current pulse abortData protocols store parameters of all delivered pulses for quality control and troubleshooting purposesStorage of over 1000 protocolsThe supplied safety dome connects to the generator to safely deliver high-voltage electrical pulses to cuvettes. Up to two cuvettes can be electroporated simultaneously.Scope of supply: Generator Gemini SC, safety dome, electroporation cuvettes 1 mm / 2 mm / 4 mm (10 each), cuvette rackExponential decay pulseCapacitance LV mode:25 ... 3275 µFCapacitance HV mode:10, 25, 50 µFResistance:50 ... 1000 ΩVoltage LV mode:10 ... 500 VVoltage HV mode:510 ... 3000 VSquare wave pulseCapacitance LV mode:3775 µFCapacitance HV mode:85 µFVoltage LV mode:10 ... 500 VVoltage HV mode:510 ... 3000 VMultiple pulse LV mode:1 ... 100 pulses per sampleMultiple pulse HV mode:1 ... 2 pulses per samplePulse length LV mode:0.05 ... 100 msPulse length HV mode:50 µs ... 5 msPulse interval:0.1 ... 10 sCharging time LV mode:7 sCharging time HV mode:4 s

Multifunctional system for fast, efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer, as well as square wave electroporation for transfection of genes and other molecules into mammalian cell lines.Large, easy-to-use touchscreen interfacePreset protocols for common hybridoma, embryo manipulation, and nuclear transfer applicationsSeparate electrofusion and electroporation function menus for optimised protocol programming, data organisation, and navigationQuick start menus for fast access to the most frequently used electrofusion and electroporation parametersUser-defined electrofusion protocols: combination of up to 19 AC protocol steps and 1 DC protocol stepStorage of up to 1000 user-defined protocolsThe supplied safety stand connects to the generator to safely deliver high voltage electrical pulses to cuvettes.The flat electrode generates a divergent field for cell fusion.Scope of supply: Generator ECM® 2001+, microslide (0.5 mm / 3.2 mm), Meander fusion chamber, flat electrode, electrode adapter, connection cable, safety stand, electroporation cuvettes 1 mm / 2 mm / 4 mm (10 each), cuvette rackSquare wave (DC)Voltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse:1 ... 100 pulses per samplePulse length LV mode:10 µs ... 999 msPulse length HV mode:10 µs ... 600 µsPulse interval:0.1 ... 10 sSine wave (AC)Voltage:5 ... 75 VFrequency:0.2 ... 2 MHzPulse length:0 ... 99 sCharging time:7 s

Multifunctional system for fast, efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer, as well as square wave electroporation for transfection of genes and other molecules into mammalian cell lines.Large, easy-to-use touchscreen interfacePreset protocols for common hybridoma, embryo manipulation, and nuclear transfer applicationsSeparate electrofusion and electroporation function menus for optimised protocol programming, data organisation, and navigationQuick start menus for fast access to the most frequently used electrofusion and electroporation parametersUser-defined electrofusion protocols: combination of up to 19 AC protocol steps and 1 DC protocol stepStorage of up to 1000 user-defined protocolsThe supplied safety stand connects to the generator to safely deliver high voltage electrical pulses to cuvettes.Scope of supply: Generator ECM® 2001+, safety stand, electroporation cuvettes 1 mm / 2 mm / 4 mm (10 each), cuvette rackSquare wave (DC)Voltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse:1 ... 100 pulses per samplePulse length LV mode:10 µs ... 999 msPulse length HV mode:10 µs ... 600 µsPulse interval:0.1 ... 10 sSine wave (AC)Voltage:5 ... 75 VFrequency:0.2 ... 2 MHzPulse length:0 ... 99 sCharging time:7 s

Multifunctional system for fast, efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer, as well as square wave electroporation for transfection of genes and other molecules into mammalian cell lines.Large, easy-to-use touchscreen interfacePreset protocols for common hybridoma, embryo manipulation, and nuclear transfer applicationsSeparate electrofusion and electroporation function menus for optimised protocol programming, data organisation, and navigationQuick start menus for fast access to the most frequently used electrofusion and electroporation parametersUser-defined electrofusion protocols: combination of up to 19 AC protocol steps and 1 DC protocol stepStorage of up to 1000 user-defined protocolsThe 0.5 and 1.0 mm microslides produce a divergent energy field ideal for efficient embryo fusion. The 3.2 mm microslide provides a homogeneous energy field for high fusion rates of hybridoma cells.Scope of supply: Generator ECM® 2001+, microslides with round wire electrode (0.5 mm / 1.0 mm), microslide with rectangular wire electrode (3.2 mm), micrograbber adapter cablesSquare wave (DC)Voltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse:1 ... 100 pulses per samplePulse length LV mode:10 µs ... 999 msPulse length HV mode:10 µs ... 600 µsPulse interval:0.1 ... 10 sSine wave (AC)Voltage:5 ... 75 VFrequency:0.2 ... 2 MHzPulse length:0 ... 99 sCharging time:7 s

Multifunctional system for fast, efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer, as well as square wave electroporation for transfection of genes and other molecules into mammalian cell lines.Large, easy-to-use touchscreen interfacePreset protocols for common hybridoma, embryo manipulation, and nuclear transfer applicationsSeparate electrofusion and electroporation function menus for optimised protocol programming, data organisation, and navigationQuick start menus for fast access to the most frequently used electrofusion and electroporation parametersUser-defined electrofusion protocols: combination of up to 19 AC protocol steps and 1 DC protocol stepStorage of up to 1000 user-defined protocolsThe coaxial chambers have identical electrical characteristics to allow direct scale-up to production once the pulse parameters have been optimised. Coaxial optimisation chamber with transparent bottom allows visualisation of the cell alignment by inverted or regular microscope.Scope of supply: Generator ECM® 2001+, coaxial optimisation chamber (2 ml), coaxial production chamber (9 ml), adapter set, cytofusion medium C (500 ml)Square wave (DC)Voltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse:1 ... 100 pulses per samplePulse length LV mode:10 µs ... 999 msPulse length HV mode:10 µs ... 600 µsPulse interval:0.1 ... 10 sSine wave (AC)Voltage:5 ... 75 VFrequency:0.2 ... 2 MHzPulse length:0 ... 99 sCharging time:7 s

Multifunctional system for fast, efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer, as well as square wave electroporation for transfection of genes and other molecules into mammalian cell lines.Large, easy-to-use touchscreen interfacePreset protocols for common hybridoma, embryo manipulation, and nuclear transfer applicationsSeparate electrofusion and electroporation function menus for optimised protocol programming, data organisation, and navigationQuick start menus for fast access to the most frequently used electrofusion and electroporation parametersUser-defined electrofusion protocols: combination of up to 19 AC protocol steps and 1 DC protocol stepStorage of up to 1000 user-defined protocolsScope of supply: Generator ECM® 2001+, high voltage output cable 1.5 m, adapter setSquare wave (DC)Voltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse:1 ... 100 pulses per samplePulse length LV mode:10 µs ... 999 msPulse length HV mode:10 µs ... 600 µsPulse interval:0.1 ... 10 sSine wave (AC)Voltage:5 ... 75 VFrequency:0.2 ... 2 MHzPulse length:0 ... 99 sCharging time:7 s

The ECM® 399 is an electroporation system for the simple transformation of bacteria and yeast cells. It produces exponential decay waves and is designed to deliver the field strengths and pulse lengths required for transformations. The ECM® 399 is ideal for basic transformation in research and academic environments. It is easy to operate, cost effective, compact in size and portable.Applications: Transformation of bacteria, yeast, fungus.Scope of supply: Generator ECM® 399 , PEP cuvette holder, Cuvettes 1 mm, 2 mm, 4 mm (10 each) and Cuvette RackSpecificationsCapacity/Resistance Range:LV Mode: HV Mode:1050 µF / 150 Ohm, fixed 36 µF / 150 Ohm, fixedMax. Voltage Output:2500 V (Peak)Pulse Interval: Charge Time:100 ms to 10 s max. 5 sMax. Pulse Length:125 ms at 500 V (Peak) or 5 ms at 2500 V (Peak)Display:16-character, liquid crystal, LCD backlitInterface: Relative Humidity:Digital User Interface 60 %Power supply:100 - 240 V, 50 / 60 Hz

The Gemini Twin Wave devices are flexible systems that can generate both square wave and exponential decay waves. Ideally suited for all electroporation requirements. The waveform combination allows eukaryotic and prokaryotic cells to be electroporated easily and efficiently in cuvettes or plates with a single device. Prokaryotic cells typically respond well to exponential decay wave pulses, while eukaryotic cells are most efficiently transfected with square wave pulses.Large, user-friendly touchscreen interfaceUniversal electroporation for transfection of cells in vitro, in vivo, in ovo, and adherent formsPreset protocols include the most common eukaryotic and prokaryotic cell typesUser-defined protocols: protocols can be added and modified without limitsSafety functions: Pre-pulse resistance check, arc protection, and over-current pulse abortData protocols store parameters of all delivered pulses for quality control and troubleshooting purposesStorage of over 1000 protocolsThe supplied safety dome connects to the generator to safely deliver high-voltage electrical pulses to cuvettes. Up to two cuvettes can be electroporated simultaneously.Scope of supply: Generator Gemini X2, safety dome, electroporation cuvettes 1 mm / 2 mm / 4 mm (10 each), cuvette rackExponential decay pulseCapacitance LV mode:25 ... 3275 µFCapacitance HV mode:10, 25, 35, 50, 60, 75, 85 µFResistance LV mode:25 ... 1575 ΩResistance HV mode:50 ... 1575 ΩVoltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse:1 ... 2 (depending on amplitude limit of the device)Pulse interval:5 ... 30 sSquare wave pulseCapacitance LV mode:3775 µFCapacitance HV mode:85 µFVoltage LV mode:5 ... 500 VVoltage HV mode:505 ... 3000 VMultiple pulse single sample:1 ... 99 pulses per sampleMultiple pulse plate:1 ... 10 pulses per samplePulse length LV mode:10 µs ... 999 msPulse length HV mode:10 ... 600 µsPulse interval:0.1 ... 10 sCharging time LV mode:7 sCharging time HV mode:4 s